Environmental Technology Research Network
in the Asia-Pacific Region

DB for Research Project
Category(1) : Hazardous substances
Category(2) : Impact assessment

1. Project Name
2. Duration
3. Research Catagory 1
4. Research Catagory 2
5. Research Field
6. Researchers
7. Affiliation 1
8. Affiliation 2
9. Address
10. Country
11. Phone
12. Fax
13. Project Summary
14. Publications
15. International Joint Study with

Mechanical Engineering Laboratory(MEL), Japan

1. Evaluation Method for Manufacturing Systems
2. 1992-1996
3. global change, air, water, wastes, hazardous substances
4. counter measures, source inventory, impact assessment, risk management
5. engineering
6. Shun'ichi SADO, Atsushi IWATA, Hideo INOUE
7. Surface and Interface Technology Division,Department of Manufacturing Systems
8. MEL, AIST, MITI
10. Japn
11. 81-298-61-7211
12. 81-298-61-7167
13. In order to reduce the ecological impact of manufacturing, it is necessary to minimize the environmental burden of each part of the system. We have developed a new computerized simulation method for assessing the environmental impact of proposed new products and manufacturing processes. Inputs to the simulator include environmental loads, and product and facility information. The materials and energy which are input to and output from the system under analysis are measured in "Ecounits", an index which represents the total environmental burden of the resources used and system outputs. The output from the simulator allows calculation of the predicted increment in ecounits caused by operation of the system. During tests of the simulator, the environmental impact of a hypothetical factory producing mechanical components was predicted, and the simulation method was shown to provide realistic and useful data.
14. 1) Shun'ichi Sado, Atsushi, Iwata, Hideo Inoue, Seiji Nakahara, Nobuo Shikata, Ecofactory Manufacturing System for Future Generations and its Evaluation Method through Computer Simulation, Environmentally Conscious Design and Manufacturing, 2, 79-84, 1994
    2) Shun'ichi Sado, Atsushi Iwata, Planning and Evaluation of Machinery Manufacturing Processes in Environmental Burden Respects, International Journal of Environmentally Conscious Design & Manufacturing, 4(1), 83-89, 1995

Hokkaido National Industrial Research Institute(HNIRI), Japan

1. Establishment of genetically engineered cells expressing human liver enzymes for novel mutagenic assay
2. 1996-1999
3. hazardous substances, mutagenic assay
4. impact assessment, risk management
5. biology, toxicology
6. Kozo Ishizaki, Satoru Ohgiya, Tamotsu Hoshino
7. Biochemistry Section, Bioscience and Chemistry Department
8. HNIRI, AIST, MITI
9. 2-17-2-1 Tsukisamu-higashi, Toyohira-ku, Sapporo 062
10. Japan
11. 81-11-857-8923
12. 81-11-857-8992
13. Most mutagenic compounds in the environment are activated by metabloic enzymes in human liver. In the conventional mutagenic bioassay systems, extract from rat liver has been used for an alternative of human liver extract. This study aims to establish yeast and mammalian cell lines that express the human metabolic enzymes by the genetic engineering and assay mutagenicity of environmental compounds with the recombinant cells.
14. 1) Satoru Ohgiya et. al., Mouse NADPH-cytochrome P450 oxidoreductase: Molecular cloning and functional expression in yeast, Biochim. Biophys. Acta, 1186, 137-141, 1994.
    2) Satoru Ohgiya et. al., Complete primary structure and fast mobility of expressed protein on electrophoresis, Biochim. Biophys. Acta, 1216, 237-244.
    3) Minoru Sawada et. al., Stable expression of guinea pig NADPH-cytochrome P450 reductase and Monkey P4501A1 in Chinese Hamster cells: Establishment of cell lines highly sensitive to aflatoxin B1, Arch. Biochem. Biophys., 300, 164-168, 1993.