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Environmental Technology Research Network
in the Asia-Pacific Region

DB for Research Project
Category(1) : Others
Category(2) : Risk management


  1. Project Name
  2. Duration
  3. Research Catagory 1
  4. Research Catagory 2
  5. Research Field
  6. Researchers
  7. Affiliation 1
  8. Affiliation 2
  9. Address
  10. Country
  11. Phone
  12. Fax
  13. Project Summary
  14. Publications
  15. International Joint Study with

Hokkaido National Industrial Research Institute(HNIRI), Japan

  1. Establishment of genetically engineered cells expressing human liver enzymes for novel mutagenic assay
  2. 1996-1999
  3. hazardous substances, mutagenic assay
  4. impact assessment, risk management
  5. biology, toxicology
  6. Kozo Ishizaki, Satoru Ohgiya, Tamotsu Hoshino
  7. Biochemistry Section, Bioscience and Chemistry Department
  8. HNIRI, AIST, MITI
  9. 2-17-2-1 Tsukisamu-higashi, Toyohira-ku, Sapporo 062
  10. Japan
  11. 81-11-857-8923
  12. 81-11-857-8992
  13. Most mutagenic compounds in the environment are activated by metabloic enzymes in human liver. In the conventional mutagenic bioassay systems, extract from rat liver has been used for an alternative of human liver extract. This study aims to establish yeast and mammalian cell lines that express the human metabolic enzymes by the genetic engineering and assay mutagenicity of environmental compounds with the recombinant cells.
  14. 1) Satoru Ohgiya et. al., Mouse NADPH-cytochrome P450 oxidoreductase: Molecular cloning and functional expression in yeast, Biochim. Biophys. Acta, 1186, 137-141, 1994.
    2) Satoru Ohgiya et. al., Complete primary structure and fast mobility of expressed protein on electrophoresis, Biochim. Biophys. Acta, 1216, 237-244.
    3) Minoru Sawada et. al., Stable expression of guinea pig NADPH-cytochrome P450 reductase and Monkey P4501A1 in Chinese Hamster cells: Establishment of cell lines highly sensitive to aflatoxin B1, Arch. Biochem. Biophys., 300, 164-168, 1993.